Fig. 3. Differentiation of confocal images revealed dye egress from depolarising mitochondria. (A) High magnification images (x63 oil-immersion objective), revealed depolarisations of individual mitochondria (arrows). (B) Differentiated images reveal only those pixels in which the signal changed between successive image frames. `Clouds' of fluorescence can be seen surrounding individual mitochondria as the TMRE moves into the cytosol. Plots of the signal with time from 3A are shown in C. Arrows indicate the depolarisations illustrated in the preceding images. Bar, 5 µm.

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