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Fig. 8. Reactivity of CD151 with antibody TS151R is cell type specific. Cryostat sections of human kidney, skin and heart muscle were processed for double immunofluorescence confocal microscopy using TS151R (A,G,J) and P48 (D) against CD151, GoH3 against {alpha}6 (E), 439-9B against ß4 (H) and polyclonal antibodies against {alpha}3 (B) or {alpha}7 (K). The composite images (C,F,I,L) were generated by superimposition of the green and red signals, with areas of overlap appearing as yellow. TS151R against CD151 reacts with CD151 in tubular epithelial cells but hardly reacts in glomerular cells (A-C), keratinocytes (G-I) and cardiomyocytes (J-L). P48 against CD151 is used in the skin for comparison and shows a prominent staining especially at the dermo-epidermal junction (D-F). Bar, 10 µm.





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