Fig. 4. Association of CD151 with 3ß1 and 6ß1 in transfected K562 cells. Transfected K562 cells stably expressing 3ß1 or 6ß1 were ¹²⁵I-surface-labeled, extracted with 1% CHAPS and lysates were subjected to immunoprecipitation with antibodies. Antibodies used were the anti-integrin antibodies J143 against the 3- and J8H against the 6 subunit and the anti-tetraspanin antibodies MEM62 against CD9, MEM53 against CD53, 6H1 against CD63, M38 against CD81, C33 against CD82 and 5C11 against CD151. Both 3ß1 and 6ß1 are present in immunoprecipitates prepared with antibodies against CD63, CD81 and CD151. However, the amount of integrin that is coprecipitated with CD151 is much greater than with the other tetraspanins, reflecting the stability and selectivity of the CD151-integrin binding. The formation of multimolecular complexes is reflected by the additional presence of CD81 (TM4) precipitated by antibodies against CD63 and CD151.

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