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Fig. 11. No complexes are formed between dystroglycan or the Lutheran blood group molecule and CD151-{alpha}3ß1 complexes in human podocytes and proximal tubular epithelial cells in culture. Human podocytes (upper panels) and proximal tubular epithelial cells (PTEC) (lower panels) expressing the laminin receptors {alpha}3ß1, dystroglycan (DG) and Lutheran blood group antigens (LU) were lysed in 1% CHAPS and precipitated with polyclonal antibodies against {alpha}3 or DG (AP83) and mAbs against CD151 (P48) or LU (LU4F2). The immunoprecipitates were analyzed by immunoblotting for the presence of {alpha}3 and DG using specific antibodies. Note that DG is not coprecipitated with {alpha}3ß1 or CD151 and that {alpha}3ß1 is not seen in the precipitates obtained by antibodies against LU or DG. {alpha}3L, {alpha}3 light chain; ß-DG, ß-dystroglycan.





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