Fig. 7. PC-GFP dynamics following injection of anti-EAGE. Cells expressing PC-FP were incubated in the presence of ascorbate for 10 minutes prior to microinjection of anti-EAGE. When imaged immediately after injection, most PC-GFP-labelled structures were immobile (A; arrowhead). A small proportion (1-5 per cell) remained motile and trafficked to the Golgi (A; arrow) and (A1-A4, which shows time lapse images of the cell in A taken 3 seconds apart) (see also Movie 4 at jcs.biologists.org/supplemental ). At later time periods after injection (30 minutes in the continued presence of ascorbate) few punctate structures remain and significant ER labelling is seen in the cells but those that are still visible are immobile (B1-B4, showing images 3s apart, arrowheads mark static structures) (see also Movie 5 at jcs.biologists.org/supplemental ). Bars=5 µm.

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