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Fig. 3. PC-GFP transport complexes do not label for ERGIC-53 or COPI during
transport to the Golgi. Cells expressing PC-GFP in the presence of ascorbic
acid were fixed 10 minutes after the addition of ascorbate and processed for
immunofluorescence with antibodies directed against COPI (B) or ERGIC-53 (D).
Note that PC-FP structures (A) do not label for COPI (B) and similarly PC-GFP
TCs (C) do not label for ERGIC-53 (D, arrows). Bars=5 µm.
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