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Fig. 8. Quantitative analysis of p65 translocation, I{kappa}B{alpha} degradation and NF-{kappa}B-dependent transcription in single living cells. (A) Dual confocal microscopy images of p65-dsRed and I{kappa}B{alpha}-EGFP at different time intervals following 1 hour LMB treatment followed by TNF{alpha} stimulation. Images are shown before and after the 1 hour LMB treatment and 40 minutes after TNF{alpha} stimulation. (B) Quantitative analysis of the time course of p65-dsRed translocation, I{kappa}B{alpha}-EGFP degradation and cell luminescence (luciferase activity) in three cells a-c (A,C) as described in Fig. 6. (C) Luminescence images of cells following TNF{alpha} stimulation. Luminescence directed by NF-{kappa}B-promoter-directed luciferase activity. Cells marked a-c correspond to those marked in A and used for quantification in B.





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