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Fig. 4. Confocal microscopy of p65-dsRed and I ${kappa}$ B ${alpha}$ -EGFP in living cells. Time series images of I ${kappa}$ B ${alpha}$ -EGFP and p65-dsRed fluorescence at stated times (in minutes) after addition of 10 ng/ml TNF ${alpha}$ . Green and red fluorescence were recorded as separate images and then merged for visualisation. Green I ${kappa}$ B ${alpha}$ -EGFP and red p65-dsRed co-localisation are represented as yellow in the presence of higher I ${kappa}$ B ${alpha}$ -EGFP:p65-dsRed ratios, and orange in the presence of higher p65-dsRed:I ${kappa}$ B ${alpha}$ -EGFP ratios.

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