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Fig. 2. Degradation of I
B
-EGFP in response to TNF stimulation.
(A) Time series images of IB-EGFP fluorescence at stated times
(in minutes) after addition of TNF. The degradation was prevented in
cells treated with 12.5 µM Bay11-7082 for 30 minutes prior to addition of
TNF. (B) Degradation of IB-EGFP in response to
TNF. Mean cellular fluorescence intensities were determined for
fluorescent cells at each time point and plotted as a percentage of the
fluorescence values at t=0 minutes. Confocal microscopy was carried
out in cells transfected with IB-EGFP 24 hours prior to their
use. Cells were treated with appropriate inhibitor for 15 minutes (for SN50
and SN50M) or 30 minutes (for Bay11-7082) prior to stimulation with 10 ng/ml
TNF at t=0 minutes. Results show mean
values±s.d.(n-1) from two experiments with at least
six cells per experiment plotted for each treatment.
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