Fig. 7. Expression of constitutively active Cdc42 blocks EC vacuole formation in three-dimensional collagen matrices. Upper panel: ECs were photographed using phase contrast (A,C) and fluorescence microscopy (B,D-F). ECs were treated with control GFP adenovirus (A,B,E) or Cdc42 CA adenovirus (C,D,F). Arrowheads indicate the presence of EC vacuoles. Shown in A-D are ECs digested from collagen assays (4 hours) and plated onto coverslips. Arrowheads indicate the presence of EC vacuoles. (E,F) Fluorescent images of intact cultures fixed at 24 hours of morphogenesis. Arrowheads indicate vacuole and lumen formation. Lower panel: magnified images of intact cultures using ECs infected with GFP, CA Rac and CA Cdc42 adenovirus at 24 hours in three-dimensional collagen matrices.

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