Fig. 5. Influence of Rho GTPase mutants on EC vacuole and lumen formation in three-dimensional collagen matrices. Endothelial cells were infected for 24 hours prior to resuspension in three-dimensional collagen matrices. Cultures were allowed to proceed for 24 (A,B) or 48 (C,D) hours in the presence (A,C) or absence (B,D) of TPA before fixation in paraformaldehyde. Quantitation was carried out using fluorescence microscopy. The average vacuole formation expressed as a percentage of cells forming vacuoles is shown±s.d. (200 cells counted per group; n=3).

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