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Kinesins klp5⁺ and klp6⁺ are required for normal chromosome movement in mitosis

Robert R. West, Terra Malmstrom and J. Richard McIntosh

Wild-type, klp5D, and klp6D cells were followed through mitosis with GFP-a-tubulin and Hoechst 33342 staining by deconvolution microscopy as described in Materials and Methods. Image stacks of eight focal planes with a 400 nm step size were collected at the indicated time intervals and rendered into a 2D image to compile a QuickTime movie. Each time point constitutes a single frame in each movie.

Wild-type mitosis. The time course starts with a cell nearing the completion of anaphase A, with the DNA elongated along the mitotic spindle. The DNA quickly becomes two equal masses along the elongating spindle. Mitosis is followed through the end of anaphase B, when the DNA reaches the poles of the cell.

klp5D mitosis. The time course starts as the DNA undergoes the first of three asymmetric divisions. Eventually the chromosome movement produces an even segregation of the DNA. The spindle elongates in anaphase B to eventually break (yellow arrow) as its constrained at the ends of the cell.

klp6D mitosis. The time course starts as one mass of DNA separates from the rest. Two subsequent chromosome movements lead to an even segregation of the DNA. The cell is followed through the end of anaphase B.

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