Fig. 6. Cell proliferation and integrin expression are altered in syndko mice during wound healing in response to dermabrasion. (A) Dermabrasion was performed on wild-type and synd1ko mouse back skin after removal of hair; and mice were sacrificed 2 and 5 days after hair removal. Synd1ko mouse have deeper and more inflamed wounds at both 2 and 5 days compared with the wildtype. (B) Cell proliferation was analyzed in unwounded mouse back skin from which hair had been removed 2 or 5 days previously and in dermabraded mouse skin at 2 and 5 days. Although the variation among both individual mice and within the same mouse after wounding is substantial, analyses of over 85 visual fields per sample for 2 days and over 30 visual fields for the 5-day samples yield data that are significant (P>0.05) at both 2 and 5 days by the unpaired t-test. Further, unwounded synd1ko and wild-type skin have significantly different cell proliferation rates at both 2 and 5 days after hair removal. (C) Colocalization of 9 integrin and ZO-1 indicates that unwounded skin from which hair had been removed 2 days previously has a normal distribution of ZO-1. Both wild-type and synd1ko mouse back skin show basal cell localization for 9 integrin. This distribution is similar to that observed in control skin taken from the lip, as shown in Fig. 1D. 2 days after dermabrasion, there is an increase in the number of cell layers expressing 9 integrin in the wild-type skin. The increase is less dramatic in the wounded skin of synd1ko mice. In both wild-type and synd1ko skin, ZO-1 localization over the involved skin areas is discontinuous. (D) Colocalization of ß4 integrin with synd1, and 3 integrin with E-cadherin, is shown in wounded skin 2 days after dermabrasion in wild-type and synd1ko mice. There are no differences in the localization of ß4 integrin, and synd1 is not present in the synd1ko tissues. 3 integrin and E-cadherin are also not differentially affected by wounding in synd1ko mice. Bar in C and D, 75 µm. (E) Immunoblots of skin tissue extracts from unwounded and dermabraded mouse skin show differences in integrin expression after wounding. Samples were normalized on the basis of their protein concentration; tissues were extracted from two mice per variable, and dermabrasion repeated twice. cont., extracts from tissues immediately after sacrifice and removal of hair; u, extracts from tissues from which hair had been removed 2 days prior to sacrifice; w, extracts from tissues from animals that had been wounded 2 days prior to sacrifice.

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