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Fig. 6. MURF2 associates with A-band titin in nascent myofibrils. C57 cells were
stained with anti-titin A-band (T31, red) and anti-MURF2 (green) antibodies.
After 24 hours of differentiation (A), titin aligned with MURF2 positive
microtubules in a dotty pattern. After 48 hours of differentiation (B), titin
A-band epitopes were organised in patches along MURF2 positive structures. At
120 hours of differentiation (C), MURF2 colocalises with titin A-band epitopes
as longitudinally oriented dots (arrowhead). Some dots were observed at both
sides of nascent striated myofibrils (arrow).