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Fig. 3. Selective association of MURF2 with post-translationally modified microtubules. (A) Transfection of full-length T7-tagged MURF2 in the myogenic cell line BHK-21 shows that microtubule-associated MURF2 (green, arrows) is excluded specifically from tyrosinated microtubules, which are stained by the YL1/2 antibody (red, arrowheads). (B) Transfected MURF2 (green) is localised on polyglutaminated microtubules in HeLa labelled by the monoclonal antibody ID5 (red; overlay yellow).





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