Fig. 9. Association of Chimeras with rafts. (A) MDCK and Caco-2 cells expressing Cav-1, Cav-2 or Cav-1 + Cav-2 were lyzed in a buffer containing Triton X-100 at 4°C and loaded in sucrose gradients. Raft and soluble fractions were harvested and analyzed by SDS-PAGE and immunoblotting with the N-20 antibody for Cav-1 (black bars) or the anti-Myc antibody for Cav-2 (empty bars) in Caco-2 cells. Blots were quantified by densitometry after scanning and the results are expressed as the percentage of protein found in the raft fraction versus the total amount of protein found in the gradient (n=3). (B) Caco-2 cells expressing CH-I to IV were treated as in A using the N-20 antibody. The results are expressed as the percentage of protein found in the raft fraction versus the total amount of protein found in the gradient (n=3). Only CH-I is found in majority in the raft fraction.

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