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Fig. 8. Oligomerization state of the chimeras in Caco-2 cells. The formation of
oligomers by Cav-1, Cav-2 and the chimeras was analyzed as in
Fig. 5 and quantified by
densitometry after scanning of the western blots using the N-20 (Cav-1 and
chimeras) or the anti-Myc antibody (Cav-2). Results are given as a percentage
of the amount of protein found in the lower part of the gradients versus the
total amount of protein detected into the gradient. (A) Quantification of the
percentage of Cav-1 (black bars) and Cav-2 (empty bars) forming high molecular
weight oligomers in Cav-1, Cav-2 and double transfected Caco-2 cells (Cav-1 +
Cav-2). (B) Quantification of the percentage of chimeras forming high
molecular weight oligomers in transfected Caco-2 cells. Cav-2 and the chimeras
are mainly found in mono- and dimeric forms as opposed to Cav-1.
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