Fig. 3. Immunoelectron microscopic localization of Cav-2 (A), Cav-1 (B) or Cav-1 and Cav-2 (C-G) in transfected Caco-2 cells. Ultrathin cryosections were stained with antibodies to Cav-1 (pAb N20) and/or Cav-2 (mAb65). (A) Cav-2 labeling appeared confined to intracellular structures (arrows). No gold particles were seen at the plasma membrane, neither at the basal (bpm) and lateral (lpm) nor at the apical (apm) plasma membrane. The star indicates apical microvilli. (B) Immunogold labeling for Cav-1 was observed at the basolateral plasma membrane (bpm, lpm). (C-G) Double immunogold labeling of Cav-1 (6 nm gold) and Cav-2 (15 nm gold) in Caco-2 cells co-expressing Cav-1 and Cav-2. (C) The two types of gold particles for Cav-1 and Cav-2 were found distributed on intracellular profiles, but only Cav-1 labeling was also observed along the plasma membrane (pm, arrowheads). (D-E) Gold particles for Cav-1 (small arrows) or Cav-2 (large arrow) were observed on vesicular or tubular membrane profiles. G, putative Golgi complex. (F-G) Immunogold labeling for Cav-1 could be seen here on the plasma membrane (pm, arrowhead), in particular on caveolae (arrows). Bars, 100 nm.

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