Fig. 6. Interaction of hARL5 and mutants with four HP1 deletion derivatives in the two-hybrid system. The diagram of HP1 and its deletion derivatives was adapted from Seeler et al., 1998 (Seeler et al., 1998) (A, left). The adapted figure was reproduced with permission from National Academy of Sciences, USA. Yeast reporter strain L40 co-transformed with pACT2-HP1 deletion derivatives and the indicated pLexA-ARL5 construct or pLexA-lamin were plated and assayed as described above (B). Panel A (right) shows the summary of the interaction result. Samples (20 µg) of transformed yeast lysates were subjected to SDS-PAGE in a 10% gel (C, upper panel). Positions of protein standards (kDa) are on the left. Proteins were transferred to nitrocellulose and reacted with anti-HA antibodies (C, lower panel), followed by detection using the ECL system. Red underlines (A) indicate regions of HP1 that interact with ARL5.

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