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Fig. 5. Chromosomal mutagenesis in Pol{kappa}-overexpressing cells. Pol{kappa} overexpression was estimated by semi-quantitative RT-PCR (A). A 30-cycle amplification led to a saturating signal (ND, not detectable), whereas a 10- or a 20-cycle procedure allowed measurement of the signal overexpression ratio between RNA extracts from untreated and doxycyclin-treated pTet-Off/pTRE2-pol{kappa}-8-4-containing cells. (B) Untransfected MRC5 cells or MRC5 cells containing either pTet-Off/pTRE2 (8-TRE2) or pTet-Off/pTRE2-pol{kappa} (8-4) plasmids were grown in the presence or absence of doxycycline and then incubated with 6-thioguanine to select hprt mutants. Errors were calculated from three independent experiments.





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