Fig. 1. Localisation of eGFP-pol in the whole MRC5 cells (A) and colocalisation of eGFP-pol with PCNA in 20% MRC5 cells corresponding to the cell population in S phase (B). MRC5 cells were transfected with plasmid peGFP-C2 or peGFP-C2/pol. Cells were then fixed, stained with anti-PCNA mAb, incubated with TRITC-conjugated secondary antibody, and analysed by using confocal microscopy for the localisation of either eGFP or eGFP-pol fluorescent proteins or PCNA. The localisation of eGFP-pol (green) and PCNA (red) was observed in the same cell and the colocalisation is indicated by the yellow pattern (Merge). About 1000 fluorescent cells were analysed. DNA content was evaluated by analysing 20,000 collected events by flow cytometry (C).

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