Fig. 3. Role of IAP and PTX in regulating TSP-1-induced cell responses. (A) Histogram shows the effect of a function-blocking IAP antibody (B6H12) on both TSP-1-induced cell chemotaxis (20 and 50 µg/ml) and DNA synthesis (50 µg/ml). Each bar represents the mean±s.e.m. of four (chemotaxis) or six (DNA synthesis) separate cell strains. Data are expressed as a percentage of the TSP-1-stimulated response (chemotaxis, 248±60 cells; DNA synthesis, 404±64 cpm), which was taken to be 100%. (B) Histogram shows the effect of pre-treatment of vascular smooth muscle cells with pertussis toxin (0.5 µg/ml for 18 hours) on both TSP-1-induced cell chemotaxis and DNA synthesis. Each bar represents the mean±s.e.m. of six (chemotaxis) or four (DNA synthesis) separate cell strains. Data are expressed as a percentage of the TSP-1-stimulated response (chemotaxis, 229±34 cells; DNA synthesis, 456±101 cpm), which was taken to be 100%.

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