Fig. 2. Effect of integrin antibodies on cellular responses. (A) Histogram shows the dose-dependent effect of the function-blocking antibody to _vß₃ (LM609, 20 µg/ml and 50 µg/ml) on TSP-1 and PDGF-BB-induced chemotaxis on collagen-coated filters. Each bar represents the mean±s.e.m. of six separate cell strains. Data are expressed as a percentage of the stimulated response (TSP-1, 606±107 cells; PDGF-BB, 775±302 cells) which was taken to be 100%. (B) Histogram shows the effect of 20 µg/ml of the function-blocking antibodies LM609 and CD29 (ß₁ integrin subunit) on TSP-1 and PDGF-BB-induced DNA synthesis. Each bar represents the mean±s.e.m of 6-9 separate cell strains. Data are expressed as a percenatage of the stimulated response (TSP-1, 884±112 cpm; PDGF-BB, 1660±410 cpm) which was taken to be 100%. (C) Histogram shows the effect of function-blocking antibodies to ₅ß₁, ₃ß₁ and ₂ß₁ on TSP-1-induced DNA synthesis. All antibodies were used at a final concentration of 20 µg/ml. Each bar represents the mean±s.e.m. of six separate cell strains. Data are expressed as a percentage of the stimulated response (TSP-1, 1041±255 cpm), which was taken to be 100%.

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