Fig. 6. Induction of JNK phosphorylation following hypo-osmotic shock. JNK1 (p46) and JNK2 (p54) were detected by immunoblotting with rabbit antisera to native JNK or with mouse antisera against phospho-JNK pJNK1 and pJNK2 (New England Biolabs), as indicated and visualized by chemiluminescence (see Materials and Methods). Cell lines varied in the time course of JNK1 and JNK2 phosphorylation. Severely mutated cell lines have higher levels of JNK1 and JNK2 than milder mutants or controls, and phosphorylation of JNK is induced faster. B, before OS; 0, start of recovery time after 5 minutes osmotic shock.

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