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Fig. 3. Different effect of osmotic shock on the keratin cytoskeleton in the five EBS-derived cell lines studied. All cell lines were subjected to hypo-osmotic shock and fluorescently stained for K14 (antibody LL001) after 4 hours recovery. (A) NEB-1 (wildtype); (B) KEB-1, (C) KEB-2, (D) KEB-3, (E) KEB-4, (F) KEB-7. Some filament fragmentation was seen in KEB-1 and KEB-2 (arrows in B and C) and clear peripheral aggregates were seen in KEB-7 (arrows in H). Bar, 10 µm.





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