Fig. 9. Disruption of the actin cytoskeleton blocks the formation of the linear arrays of caveolin-1-GFP induced by nocodazole. CHO cells stably expressing caveolin-1-GFP were plated on coverslips and grown for 2 days, as described in Materials and Methods. Cells were incubated for 30 minutes in the absence (A,B) or presence of either 2.5 µM cytochalasin D (C,D) or 1 µM latrunculin A (E,F) and then incubated for an additional 60 minutes with (B,D,F) or without (A,C,E) 10 µM nocodazole, before fixing with paraformaldehyde and processing for visualization of GFP. Note that both cytochalasin D and latrunculin A blocked the redistribution of caveolin into linear arrays (compare B with D and F) and that nocodazole blocked the latrunculin A induced redistribution of caveolin-1-GFP to the cell center (compare E with F). Each image is the projection of a stack of confocal images that extend through the entire cell. Bar, 20 µm.

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