Fig. 8. Nocodazole reorganizes caveolin-1 and caveolae at the cell surface of normal human fibroblasts. (A-D) Normal human fibroblasts were grown on coverslips for 2 days. One set of cells was processed without further treatment for indirect immunofluorescence localization of caveolin-1 (A) and tubulin (B), while the other set was incubated in the presence of 40 µM nocodazole for 2 hours before staining for caveolin-1 (C) and tubulin (D). The linear arrays in the treated cells were both more extensive and thicker. (E) Normal human fibroblasts were grown in 6-well dishes for 2 days, incubated in the presence of 40 µM nocodazole for 2 hours, and processed for EM. This treatment induced a striking increase in the number of caveolae that could be detected by electron microscopy. Bars, 37 µm (A,B); 23 µm (C,D); 0.8 µm (E).

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