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Fig. 7. Association of caveolin-1-GFP with the actin cytoskeleton. Cells stably expressing caveolin-1-GFP were plated on coverslips, grown for 2 days and treated with 10 µM nocodazole for 30 minutes before fixing with paraformaldehyde. Panel A shows the distribution of caveolin-1-GFP; panel B the distribution of F-actin stained with Alexa-633-phalloidin; and panel C a merged image. The arrows indicate regions where caveolin is aligned along or adjacent to stress fibers.