Fig. 6. Nocodazole blocks the movement of caveolin-1-GFP in live cells and organizes caveolin-1-GFP into linear arrays. CHO cells stably expressing caveolin-1-GFP were grown for 2 days before being viewed by confocal microscopy, as described in Materials and Methods. (A) Still from video of CHO cells pretreated with 10 µM nocodazole for 1 hour before viewing. Long range movements have disappeared. (B) Immunofluorescence micrograph of CHO cells pretreated with nocodazole taken at the bottom surface of the cell. (C) Still from video of stably expressing CHO cells pretreated with 10 µM nocodazole for 1 hour before the addition of 1 µM latrunculin A. Within 1 minute the arrays have disappeared and everything appears to be immobile. Bar, 10 µm. The movies that correspond to panels A and C can be viewed online (http://jcs.biologists.org/supplemental).

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