Fig. 4. Caveolin-1-GFP displays three different types of movement. Cells were plated 48 hours before use into culture dishes that had a glass coverslip glued to the bottom. Confocal sections were taken at 1.6 second intervals near the bottom of the cell, just under the nucleus in cells stably expressing caveolin-1-GFP. (A) Some of the caveolin-1-GFP in these cells showed only saltatory movements (yellow arrows). A 12.8 second recording showed, however, that other patches of caveolin-1-GFP in the same cell rapidly moved apart (follow white arrows). (B) A third population of caveolin-1-GFP was associated with dynamic tubular structures (yellow arrows), which can also be seen in the accompanying movie. (C) Stills from two videos showing examples of long range and saltatory movement in control cells. Bar, 10 µm (A); 5 µm (B). The movies that correspond to panels B and C can be viewed online (http://jcs.biologists.org/supplemental).

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