Fig. 3. Caveolin-1-GFP is localized at the MTOC, in close proximity to recycling endosomes. CHO cells stably expressing caveolin-1-GFP were plated on coverslips and processed for confocal imaging. Cells were then fixed and labeled either with anti-mannosidase II to stain the Golgi (B), anti-tubulin to stain microtubules (E) or anti-transferrin receptor antibody to stain recycling endosomes (G). Secondary antibodies were either Alexa-568-conjugated goat anti-rabbit IgG or Alexa-595-conjugated goat anti-mouse IgG. The green channel shows the distribution of caveolin-1-GFP (A,D,G) and the red channel the distribution of mannosidase II (B), tubulin (E) or the transferrin receptor (H). Merged images are shown in panels C, F and I. Each picture is a single confocal image through the center of the cell. Bar, 10 µm.

Return to article