Fig. 10. Latrunculin A causes a redistribution of caveolin-1-GFP in CHO cells (A) and caveolae in normal human fibroblasts (B). (A) CHO cells stably expressing caveolin-1-GFP were treated with latrunculin A (1 µM) and the cells were imaged within 1 minute using confocal time-lapse microscopy. The three panels in A are stills from videos taken at 1.6 second intervals for a total of 6 minutes each after 1 minute, 10 minutes or 30 minutes of latrunculin A treatment. Within 1 minute there is a dramatic increase in the trafficking of caveolin-1-GFP in the cells. By 10 minutes the caveolin-1-GFP has formed larger aggregates and caveolin can be seen moving into and out of long processes that keep the cells attached to the substratum (arrows). By 30 minutes there was an accumulation of caveolin in the center of the cell. The videos taken at 1 minute and 10 minutes after latrunculin treatment can be viewed online. (B) Normal human fibroblasts were grown for 2 days and then incubated with 1 µM latrunculin A for 30 minutes before processing for immunogold localization of caveolin-1. Numerous large caveolin-positive structures were seen that appeared to have multiple invaginated caveolae all around them (^*). The inset shows a higher magnification of these structures. Bars, 10 µm (A); 0.24 µm (B); 0.16 µm (inset). The corresponding movies can be viewed online (http://jcs.biologists.org/supplemental).

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