Fig. 1. Osteoclastogenesis is impaired in Jnk1^-/- but not Jnk2^-/- mice. Bone marrow hematopoietic precursors were isolated from Jnk1^-/- (A,C,E), Jnk2^-/- (B,D,F) and wild-type littermates and co-cultured with wild-type primary osteoblasts in osteoclastogenic conditions. Osteoclastogenesis was monitored by TRAP staining (red color). (A,B) TRAP staining after 6 days of culture; the arrows indicate multinucleated TRAP-positive cells. (C,D) Time course of osteoclast differentiation. (E,F) Osteoclast activity measured by pit assay on bovine cortical bone slices. The method for statistical analysis is described in the materials and methods, the stars indicate the statistical significance: (^***) P<0.0001, (^**) P<0.001, (^*) P<0.005, (n) number of slices analyzed.

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