Fig. 4. PTP-PEST impairs membrane ruffling and motility in response to PDGF. (A) Parental Rat-1 fibroblasts or Rat-1 cells stably overexpressing PTP-PEST were serum starved (left panels) and then stimulated with PDGF-BB (20 ng/ml, 15 minutes, right panels) to assess membrane ruffling. Whereas control cells reorganize actin into ruffles, those that express PTP-PEST show less prominent membrane ruffling. (B) Parental Rat-1 fibroblasts or those stably expressing PTP-PEST were added to transwell filters in which the bottom membrane was coated with FN in the presence or absence of PDGF-BB in the lower chamber. While control cells are induced to migrate in the presence of PDGF, PTP-PEST-expressing cells exhibit reduced motility in response to PDGF.

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