Fig. 4. The localisation of emerin mutants through mitosis by immunofluorescence microscopy. To show simultaneously the location of exogenously expressed mutant forms of emerin and endogenous lamin A/C during mitosis, COS-7 cells were transfected with wild-type (A), missense S54F (B), Del236-241 (C) and 1-220 (D) EGFP-emerins and immunolabelled for lamin A/C. The chromatin was stained with DAPI to identify the stages in the cell cycle. The cells expressing wild-type and mutant EGFP-emerins, except for Del236-241, show a similar mitotic distribution to that of endogenous lamin A/C. The cells expressing the emerin mutant Del236-241 show an altered localisation pattern for both the EGFP-emerin and endogenously expressed lamin A/C. Cells transfected with EGFP-emerin 1-220 were similar to those transfected with EGFP-vector alone. Nikon type 108 microscope; bar, 10 µm.
