Fig. 1. Purification of recombinant S100A13. (A) Recombinant S100A13 was purified using the pPROEX HTb expression system and Ni-NTA affinity chromatography. 20 µl of protein extract from transformed DH5 cells was loaded before (lane 1) and 3 hours after (lane 2) IPTG induction. Lane 3 shows the purified fusion protein S100A13 from the Ni-NTA column. Protein extract was loaded on a 14% SDS Tricine-PAGE under reducing condition, followed by Coomassie G-250 staining. (B) Purified S100A13 from the Ni-NTA column is shown in lane 1. The fusion protein was then digested by TEV protease to remove the histidine tag as shown in lane 2, and S100A13 purified by gel filtration is shown in lane 3.

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