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Fig. 5. TPA-stimulated traffic of PKC
in control RBL and RBL-Syt
II- cells. Upper panel: control (empty-vector-transfected) RBL
cells (A) and RBL-Syt II- (B) cells were treated with TPA (50 nM)
for the indicated time periods. Cell homogenates were prepared, and equal
amounts of protein fractionated on continuous sucrose gradients as described
in the Materials and Methods. Fractions were collected from the top, subjected
to SDS-PAGE and immunoblotted with anti-PKC antibodies. The intensities
of the bands corresponding to PKC were quantified by densitometry and
are presented as a percentage of the total amount of enzyme (C). Data
represent one of two separate experiments.
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