Fig. 1. Immunofluorescence images of spindle formation in polyspermic zygotes of
S. lomentaria. (A,C,E,G) DIC images; (B,D,F,H) merged images of
immunolocalization of ß-tubulin (green), centrin (red) and DAPI staining
(blue). (A,B) Just before mitosis. Two pairs of centrioles derived from male
gametes locate at one side of the nucleus and duplicate there. Therefore, four
anti-centrin-positive spots can be observed (arrow in B). (C,D) Migration of
four centrosomes to the future mitotic poles. Arrowheads in D show
anti-centrin-positive spots. (E,F) Tri-polar spindle is formed and one of the
three poles has two centrosomes. Arrowheads in F show four anti-centrin
positive spots. (G,H) Tri-polar spindle is formed. One of the four centrosomes
is positioned away from the spindle. Arrowheads in H show four
anti-centrin-positive spots.
