Fig. 1. Sequence alignment of TbGpi8 with Gpi8 sequences of other species
and hydrophobicity plot of TbGpi8. (a) TbGpi8 protein
sequence of Trypanosoma brucei aligned with homologues of
Leishmania mexicana, Plasmodium falciparum, Saccharomyces cerevisiae,
Schizosaccharomyes pombe and Homo sapiens. Amino acid identities
and similarities are highlighted. TbGpi8 shows one potential
N-glycosylation site at position 25 (NKT, overlined), which is not conserved
in other Gpi8 molecules. In addition, TbGpi8 has a putative
hydrophobic N-terminal ER-directing signal sequence (underlined), but shows no
hydrophobic membrane-spanning region at its C-terminus when compared with
other Gpi8 molecules. (b) Hydrophobicity plot of TbGpi8 using a window of 6
amino acids: note that there is no putative transmembrane domain on the
C-terminus of the protein.
