Fig. 4. CaM-EGFP redistribution in relation to kinetochores and microtubules in
MDCK. (A) Metaphase MDCK control (no drug). (B) Star-like structures in
nocodazole (0.1 µg/ml, 4 hours)-treated MDCK. The control cell shows the
calmodulin (green) concentrated at the spindle pole in a ring-like shape, the
microtubules (red; overlapping green and red seen as yellow) and the
kinetochores (white, false colour) at the metaphase plate (blue). The
star-like structure in stage III of mitotic spindle disruption seen with four
colours gives the detailed arrangement of the four elements (B, detail shown
in inset i): calmodulin as a core with radiating microtubules and the
corresponding kinetochores attached to chromosomes. (C) CaM-EGFP
redistribution relative to kinetochores induced with nocodazole in stage IV of
spindle disruption. The kinetochores (red) are situated in immediate proximity
to the CaM-EGFP (green) accumulation and chromosomes (blue). Bar, 10
µm.
