Fig. 2. MAP1B-P expression is concentrated in extending neurites. (A) PC12 cells exposed to NGF for five days were fixed and stained with mAb SMI-31, which recognises MAP1B-P and a nuclear epitope unrelated to MAP1B (red), a MAP1B pAb (green) and YL1/2, a mAb specific for tyrosinated tubulin (blue), which stains all neurites and growth cones. MAP1B-P expression is concentrated in the longer neurites of the PC12 cells (arrowheads) and is undetectable in many shorter processes (arrows). By contrast, MAP1B is distributed throughout the cell, including the cell soma. (B) PC12 cells exposed to NGF for three days were fixed and stained with anti-GSK3ß antibodies (red), a MAP1B pAb (green) and YL1/2, a mAb specific for tyrosinated tubulin (blue). GSK3ß is expressed throughout the cell soma, neurites and growth cones.
