QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)

Fig. 6. ß1PIX induced the formation of microvillus-like structures by activating Cdc42. (A) The formation of microvillus-like structures was blocked by Cdc42^N17 but not Rac1^N17. Scanning electron micrographs (SEMs) of HeLa cells microinjected with various plasmid DNA. The DNAs were pXJ-GST vector, pXJ-Flag-ß1PIX, pXJ-Flag-ß2PIX, pXJ-HA-CDC42^V12, pXJ-Flag-ß1PIX plus pXJ-Flag-Rac^N17 and pXJ-Flag-ß1PIX plus pXJ-Flag-Cdc42^N17. The apical surface structures induced by ß1PIX are unlike the filopodia induced by Cdc42^V12. (B) PIX mutants did not induce microvillus-like structures. SEMs of HeLa cells microinjected with plasmid constructs of the PIX mutant constructs pXJ-Flag- ${Delta}$ N80ß1PIX, pXJ-Flag-ß1PIX-DHm and pXJ-Flag-ß1PIX^1-555. (C) The morphological effect of localized activation of Rac1 and Cdc42. Apical protrusions induced by ß1PIX are a combination of microvillus-like structures and lamellipodia (marked by arrowheads), seen here as higher-magnification SEM. Scale bar, 10 µm.

Return to article