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Fig. 2. Localization of FP-tagged PP1 isoforms in transiently transfected HeLa cells. Panel A shows the localization of EYFP-PP1{alpha} in live HeLa cells, with an accompanying DIC (differential interference contrast) image in Panel B and the two images merged in Panel C. EGFP-PP1ß/{delta} localization is shown the same way in Panels D-F, and EYFP-PP1{gamma} localization in Panels G-I. PP1{alpha} and PP1{gamma} maintain their specific localization patterns when co-expressed in the same cells as ECFP-PP1{alpha} (Panel J) and EYFP-PP1{gamma} (Panel K). Panel L shows the two patterns merged, with ECFP-PP1{alpha} pseudocolored green and EYFP-PP1{gamma} pseudocolored red. Arrowheads indicate nucleoli. Scale bars are 10 µM.





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