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Fig. 8. Effect of the Bcl-2 mutants on the breakdown of the mitochondrial membrane potential after CD95 (A) or TRAIL (B) stimulation. Cells were stimulated for the indicated time points, and the mitochondrial potential ( ${Delta}$ ${Psi}$ _m) was determined using the mitochondrial potential-specific dye TMRE. The amount of cells with a low ${Delta}$ ${Psi}$ _m was quantified by FACS analysis. When overexpressed at mitochondria or as wild-type protein, Bcl-2 delayed ${Delta}$ ${Psi}$ _m breakdown. However, after 24 hours almost all cells displayed a low ${Delta}$ ${Psi}$ _m irrespective of the Bcl-2 status. Bcl-2/ER influenced the breakdown of ${Delta}$ ${Psi}$ _m only modestly, whereas Bcl-2/ ${Delta}$ TM had no effect compared with vector control cells. The error bars indicate the standard deviations from independent measurements of the same cell batch.

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