Fig. 3. Caspase activation after irradiation of Jurkat cells expressing different Bcl-2 mutants. Western blot analyses were performed with cell lysates prepared 14-20 hours after irradiation with 10 Gy. The blots show that activation of caspase-9, caspase-3 and caspase-8 as well as PARP cleavage was abrogated by overexpression of either mitochondrial (Bcl-2/MT), endoplasmic (Bcl-2/ER) or wild-type Bcl-2 (Bcl-2/WT). No differences in caspase activation were detectable when Bcl-2/
TM cells were compared with vector control cells.
