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Dis1/TOG universal microtubule adaptors - one MAP for all?

Hiroyuki Ohkura1, Miguel A. Garcia2 and Takashi Toda2

1The Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, The University of Edinburgh, Edinburgh EH9 3JR, UK
2Laboratory of Cell Regulation, Imperial Cancer Research Fund, PO Box 123, 44 Lincoln’s Inn Fields, London WC2A 3PX, UK

QuickTime Video JPEG Image

Movie1

Live images of Alp14-GFP during interphase. Live images of Alp14-GFP during interphase are shown in this movie. Four interphase cells, in which two are in late G2 phase (left), whereas the other two (right) are in post-anaphase and G1 phase, are shown. Rich medium containing 2% agar was solidified on a glass slide and one drop of the culture of a strain containing the chromosomal alp14+-GFP was placed top of the agar and sandwiched with coverslip. Live images were taken at room temperature with a Zeiss Axioplan equipped with a chilled video CCD camera (C4742-95) and the PC computer containing kinetic image with a Zeiss LSM510 laser scanning confocal microscope.



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