
Fig. 4. GFP-DdRab11 co-localizes with the 100 kDa proton pump subunit in the
contractile vacuole. Cells expressing CV-localized GFP-DdRab11 were observed
and photographed (A,B). Co-localization experiments in cells expressing
GFP-DdRab11 are shown in C-E. (C) GFP-DdRab11; (D) Texas Red-tagged antibody
staining of the 100 kDa ATPase proton pump protein. (E) A dual fluorescent
image of both C,D that demonstrates complete co-localization of GFP-DdRab11
and the 100 kDa ATPase proton pump protein. Scale bar: 5 µm.