Fig. 12. Cells expressing DdRab11N125I are normal in endocytic uptake and release
rates but are enhanced in the rate of phagocytosis. DdRab11N125I (+) cells
were incubated in HL5 medium containing FITC-dextran (1 mg/ml). At the
indicated times (top), cells were recovered and washed by centrifugation and
accumulated FITC-dextran was measured using a spectrophotometer. In addition
(middle), after 3 hours of accumulation, cells were washed and resuspended in
growth medium. At the indicated times, cells were washed and the FITC-dextran
remaining intracellular was measured. Finally (bottom), cells in growth medium
were incubated with fluorescent latex beads (at the indicated times), the
cells were washed, and accumulated beads were measured using a
spectrophotometer.
