Fig. 9. Effect of XMog1 and RanBP1 on loading of Ran with guanine nucleotides by RCC1. (A) Ran (50 pmol) preloaded with unlabelled GTP was incubated with [³H]GDP for 10 minutes with addition of the specified amount of RCC1, either without XMog1 (open symbols) or with 300 pmol XMog1 (closed symbols). (B) Ran (50 pmol) preloaded with unlabelled GTP was incubated with [³H]GDP for the specified time with 1.8 pmol RCC1 and further additions of buffer (open squares), 300 pmol XMog1 (closed squares), 18 pmol RanBP1 (open triangles), or XMog1 and RanBP1 together (closed triangles). (C) Ran (50 pmol) preloaded with unlabelled GDP was incubated with [³H]GTP for the 10 minutes with addition of the specified amount of RCC1 and further additions of buffer (crosses), 300 pmol XMog1 (open circles), 18 pmol RanBP1 (open squares), or XMog1 and RanBP1 together (closed squares). In all assays the reaction volume was 50 µl.

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