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Fig. 8. Effect of XMog1 and RanBP1 on RCC1-mediated guanine nucleotide release. Ran
(50 pmol) preloaded with [3H]GDP was incubated with 1.8 pmol RCC1
for the specified time in a reaction volume of 50 µl containing 700 µM
GDP (A) or 700 µM GTP (B) before determining the amount of radioactivity
remaining bound to Ran. Additions were buffer alone (open squares), 300 pmol
XMog1 (closed squares), 18 pmol RanBP1 (open triangles) or XMog1 and RanBP1
together (closed triangles).
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